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The LTM network was also optimized temporally using the method as detailed in "Temporal optimization of the LTM Network".
These were examined histologically for evidence of degenerative change following a similar method as detailed in a previous paper by Insall [7].
Relative mRNA expression was determined using the ΔΔCT method, as detailed by manufacturer guidelines (Bio-Rad).
For the Correlation part we used the histograms method as detailed in the Methods Section.
Purification was carried out in a batch method as detailed in the manufacturer's instructions.
We made predictions of HPTFs using the method as detailed in the materials and method section.
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The current methods were validated in accordance with the guidelines recommended by the US Food and Drug Administration FDAandand the International Conference on Harmonisation (ICH) [18, 19] for analytical procedures and methods, as detailed below.
ChIP-seq was either by single- or paired-end methods as detailed in the Extended Experimental Procedures.
All 195 samples were aligned against the reference data using AGEP, NN and SVM methods, as detailed above.
The methods, as detailed below, were inspired by the decoding of neural auditory cortical activity recorded in monkeys in response to naturalistic sounds (Kayser et al., 2012).
Simulations were used to repeatedly sample from the generated "gold standard" STH data set following the protocol of alternative survey methods, as detailed below and summarized in Table 1.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com