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Acute toxicity was carried out as reported according to lorke's [27] method as describe by Amos et al., [28].
To determine the effect of storage period, experimental material batches from each of the curing treatment levels were sampled at 12, 24, and 36 weeks storage period using core sampling method as described by Rotz and Abrams (1988) and taken for CP analysis following Macro-Kjeldahl Method as describe by Association of Analytical Chemists (AOAC 1990 20055).
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Reducing sugars were analyzed by DNSA method as described above.
Such a method is called a projection method, as described elsewhere [13 17].
Intracellular RNA content was measured by PCA method as described in "Materials and Methods" section.
Cell viability was measured by the MTT method as described in materials and methods.
Parasites were synchronized with the sorbitol method as described [14].
CSE was produced following the method as described before [16].
The data was normalized using the print tip loess method as described in the methods section.
SCCmec type assignment was performed using a multiplex PCR method, as described previously [22].
More recently we have collected volatiles using the SPME method as described here.
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