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The convergence behavior of the T-matrix method as calculated by the extended boundary condition method (EBCM) is studied, in the case of light scattering by spheroidal particles.
Relative miRNA expression was normalized against the endogenous control snoRNA234 using the comparative delta-delta CT method as calculated using SDS RQ manager v1.2 software.
Cycle threshold (C(T)) values were determined by the second differential maximum method as calculated by the Rotor-Gene software.
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Normalized median differences pertain to the median value of all differences between the sample outcomes of the two methods as calculated in terms of percentage by [ y − x)/ y + x)]/2 [ 9].
The inter-laboratory reproducibility of the methods as calculated by Cohen's kappa on the quadruplicate samples of each strain is depicted in Table 2. Cohen's kappa values could not be calculated for MH-van for VSSA, MH-van for hGISA and Etest for GISA due to unbalanced variance of components.
Since the C. crescentus genome is GC-rich (67%), traditional quantitative methods such as calculating mean coverage on genes or RPKM (Reads Per Kilobase per Million mapped reads) may reduce the accuracy of gene expression quantification [ 1].
27 This type of consensus profile can be constructed from a set of known binding sites for a certain structural property by many different methods, such as calculating the average profile, averaging specific regions, Fourier analysis or error minimization.
Figures 6, 7 and Table 1 describe the relationship between % in vitro release using the 'modified dialysis' method and the % absorption, as calculated by the Nelson Wagner method and fractional AUC.
Then, genetic similarities among population samples were displayed using the Unweighted Pair-Group Method with Arithmetic Mean (UPGMA), as calculated in MEGA 4 [ 45].
The sensitivity, specificity, positive and negative predictive values of this PCR method were calculated as 88%, 100 %, 100, and 83%, respectively [ 49].
However, as calculated in Methods, we estimate that there were on average 20 µ RNA molecules per cell in the variegated clones such as #651 and #654 that produced ∼2% as much μ RNA as #626.
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