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The intervention has in Finland been taken into clinical use, but randomised trials of the effectiveness of the method are yet almost lacking.
Although, all the parameters that influence the various aspects of this method are yet to be understood completely, RNA-seq undoubtedly is playing a very important role in deciphering the complexity of the transcriptome by giving a new direction to isoforms, allelic expression, untranslated regions, splice junctions, antisense regulation and intragenic expression [ 10, 16, 29, 68- 74].
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A transferable evaluation method is yet to be developed.
For composite bonding, such test method is yet to be developed.
Multiple TACE protocols are used throughout the world, and the optimal method is yet to be established.
However, the method is yet to become a mainstream application due to a number of economical and design related issues.
False positives due to "off-target" effects of RNAi are a notorious problem in siRNA screens in human cells with current technology, and no completely satisfactory method is yet available to eliminate them.
The best method is yet unknown, but analyses comparing screening methods with efficacy can be informative.
Nonetheless, AEE estimates should be interpreted with some caution, since the method is yet to be validated against doubly labeled water in pregnant African women.
Still, in the field of Natech accident investigation these methods are yet to take the commonplace.
However, VAUS and regular adaptive umbrella sampling (AUS) methods are yet computationally expensive.
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