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I L: Writing of manuscript, development of slice method and viability test.
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Hepatocytes were isolated with two-step collagenase perfusion method and the viability of the cells was over 80%.
Hepatocytes were prepared using a two-step collagenase perfusion method, and their viability was confirmed to be >95% by the trypan blue exclusion method.
Cells were treated at slightly above a pre-determined IC50 (see Methods S1) and viability measured.
This global biodiversity hotspot is a perfect study area to test orchids for collecting methods and assessing viability.
The adhesive properties of Aeromonas hydrophila LOCK0968 were studied in water with a low concentration of organic compounds, using luminometric and microscopic methods, and the viability of the adhered bacterial cells was evaluated using the colony forming units method.
The viability of the harvested macrophages was evaluated with the trypan blue exclusion method, and the cell viability was calculated from the cell population.
Furthermore, we analyze the expression level of selected cell adhesion-related genes by the quantitative real-time (qRT-PCR) method and the cell viability for MWCNT powders.
Viable cells were counted with an inverted microscope using the trypan blue method and, typically, the viability of cells was ⩾93%.
However, the effects of potassium dichromate and bleach on the Cyclospora oocysts used in this study are unknown, since methods to evaluate infectivity and viability were not available.
The statistical error analysis method is used to examine the accuracy and viability of used methods.
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