The bubble interface is captured by a diffuse interface method and treated as a mixing region of the liquid and gas phases.
gDNA extracted with the standard phenol⁄chloroform method and treated with RNase A was then precipitated with 5.2 M ammonium acetate and dissolved in 20 30 μl HPLC water.
Total RNA was prepared from frozen cells using the TRizol (Invitrogen) method and treated with DNase I (Fermentas) as described previously [32].
RNA isolation was carried out using trizol method and treated with RNase free DNase. 2 μg of RNA was used to make cDNA and to check the telomeric transcription levels.
HAUSP-V5, FLAG-Cry1, and Actin were detected by IB in IPs or whole cell lysates (WCL) from 293T cells transfected with the indicated plasmids (by calcium phosphate method) and treated with doxorubicin.
Two subproblems, i.e. the Vlasov Poisson problem and homogeneous Landau problem, are obtained through time-splitting methods, and treated separately by the Runge Kutta Discontinuous Galerkin method and a conservative spectral method, respectively.
Recently, some researchers began to try different numerical methods and treated nanofluid as a multi-phase mixture, e.g. Eulerian [30,31] and Lagrangian approaches [32,33].
Some producers now use machines, but the most prestigious alchemist houses still apply traditional methods and treat their wines like babies in the hands of skilled workers, to make sure the end-product clings to the pride of the name.
Murine bone marrow cells were collected from femurs (see later section in Methods) and treated with colcemid (6 µl/mL in 0.075 M KCl) for 2 hours.
Thus, we prepared liposomes of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), as described in Materials and Methods, and treated them with preformed CuII-stabilized oligomers of Ub.
