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The chimeric cDNA of tPA was constructed by Splicing by Overlap Extension Polymerase Chain Reaction (SOEing-PCR) method and transferred to the hairy roots of tobacco using different strains of Agrobacterium rhizogenes.

Graphene film can be prepared ultrathin (<100 nm) by filtration method and transferred to a flexible polyethylene terephthalate (PET) substrate to make flexible supercapacitor electrodes as Fig. 11 shows [76].

Eggs were prepared with standard bleaching method and transferred to the plates.

The construct harboring 35S- SaSce9, was introduced into Agrobacterium by freeze thaw method and transferred into wild type Columbia ecotype of Arabidopsis by floral dip method [ 59].

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Subsequently, this study further applies the efficiency coefficient analysis method and transfers the score for respective variables into centesimal system points (Table 3).

The entire procedure is straightforward, and thus the resultant mode shapes are continuous unlike the result of existing methods such as the finite element method and transfer matrix method.

The objective of this article is to describe the design method of a dual-shell car horn (DSCH) with good interior sound quality based on the boundary element method and transfer path analysis (TPA).

Sixteen-week-old clones were cultured in vitro (T0), as described in Methods, and transferred for five weeks to two different in vitro growth systems: semisolid in Magenta boxes (M) and temporary immersion in modular Bioreactors (B).

Developing new LC-MS methods and transferring existing methods between instruments and laboratories are time consuming and challenging, mostly because of the large number of LC and MS parameters that require optimization.

Equal amounts of protein samples (50 µg) were resolved by SDS-PAGE on 12% gels by the method of Laemmli, and transferred to polyvinylidene fluoride membranes (PVDF) BIO-RAD Laboratoriess). BIO-RAD Laboratories

The design takes full advantage of the powerful Monte-Carlo method and transfer learning technique.

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