Data analysis will be done in accordance with guidance given for each distinct data collection method, and thereafter aggregated for final results.
The extracts were isolated by solvent maceration method and thereafter subjected to antiproliferative activity test on five different cancer cells: human colon carcinoma (HCT-116), breast carcinoma (MCF-7 and MDA-MB 231), ovarian carcinoma (SKOV-3 and COAV), and a normal liver cell line (WRL 68).
Intensity values were determined from each of the two channels using the Rosetta Resolver Ratio-Split method [55], [56] and thereafter used as single values per sample.
PTH was measured by an immunoradiometric assay (IRMA, manual method) before November 2001 and thereafter by an immunochemical luminometric assay (Nichols Advantage Specialty System).
Amaefule et al. (1993) introduced FZI and RQI methods for rock typing and thereafter these methods have been used widely in the literature (Gunter et al. 1997; Porras and Campos 2001; Jennings Jr and Lucia 2003; Rincones et al. 2000; Soto et al. 2001).
The urine was analyzed for creatinine, using the Jaffé method (Roche Diagnostics) before June 2004 and thereafter with an enzymatic method (photometry, Modular P, Roche and CREAplus R1, R2, Roche Diagnostics, Mannheim, Germany), and calcium, using photometry (Modular, Roche and Ca R1, R2, Roche Diagnostics, Mannheim, Germany).
The number of neurons in the hidden layer is chosen by trial and error method beginning from minimum and thereafter augmented reliant on the nature of problem (Gorashi and Abdullah 2012).
Women, who met the inclusion criteria, were briefed about the purpose, method and principles of confidentiality, and thereafter a suitable time for interview was arranged.
The urinary albumin concentration was determined by immunonephelometry (Beckman Instruments, CA, USA) until 1998 and thereafter by an immunoturbimetric method (Beckman Coulter, Beckman Instruments, CA, USA).
Quantitation of CD4 lymphocytes was performed using a manual system (Cytosphere, Coulter, Hialeah, Florida, USA) from March 2004 until October 2004, and thereafter by an automated method (FACS Count, Becton Dickinson, Forest Lakes, New Jersey, USA).
Total cholesterol was measured until April 1992 by the ferric chloride/acetic acid-sulfuric acid technique (Technicon AutoAnalyzer) and thereafter by the enzymatic method, which was also used for measuring HDL cholesterol.
