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Recently, high-throughput screening methods, such as the yeast-two-hybrid method and tandem affinity purification, have generated large datasets of protein-protein interactions (2, 3).
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Methods and Results: Tandem repeat motifs of 3 6 bp were searched using a simple sequence repeat (SSR)–pipeline package in coding portions of the date palm draft genome sequence.
Good correlations were obtained between the results of the developed method and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
We describe the concept and methods of peptide ligation and tandem peptide ligation for preparing synthetic and natural biologicals.
The urine samples were assayed between 2009 and 2010 for the oxidatively modified guanine nucleosides 8-oxodG and 8-oxoGuo using a validated method of ultraperformance liquid chromatography and tandem mass spectrometry (11).
The urine samples, stored at −80°C until analysis, were assayed between 2009 and 2010 for the oxidatively modified guanine nucleosides 8-oxodG and 8-oxoGuo using a validated method of ultraperformance liquid chromatography and tandem mass spectrometry (9).
The eIF3g core subunit was not detected using MALDI-TOF in this purification, but was detected in an independent purification of the Csn7Bp complex using the more sensitive method of nano-liquid chromatography and tandem mass spectrometry (LC-MS/MS, see Fig. 5A).
Two analytical methods (HPLC-fluorimeter [HPLC-FLD] and tandem mass spectrometry LC/MS/MS) are available to assay phenyl-hydroxyethylmercapturic acids (PHEMAs), the mercapturic acids of styrene in humans.
We aimed to develop a new and rapid method utilizing tandem mass spectrometry for the direct assay of the intact conjugated vitamin E metabolites and to investigate whether there was evidence for increased urinary concentrations of α-TL conjugates under conditions where oxidative stress may be implicated, such as diabetes mellitus [10,11].
For further details of the Difference in-Gel Electrophoresis (DIGE) and tandem mass spectrometry methods used (see Additional File).
Our repeatability (interday RSDs, 4.3 28 %) is comparable or better than that of another similar method [ 15] and with a method using tandem mass spectrometry (interday RSDs, 4.1 17.7 %) [ 35].
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