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The slides of MCF7 cells were prepared by a cytospin method and stained with Wright Giemsa solution (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) and photographed.
Mature parasites were enriched using magnetic column method and stained with the Fluo-4-AM as previously described for P. falciparum.
The wound tissues with normal skin adjacent to wound site were fixed in 10% of phosphate buffered formalin and processed for routine histological method and stained with a general routine stains (haematoxylin and eosin) and examined under light microscope.
One stool specimen from each patient was examined for Cyclospora spp. by microscopy of fecal materials that were concentrated by the formalin-ethyl acetate sedimentation method and stained with the modified acid-fast staining technique (1 ).
A paraffin sections were obtained by routine method and stained with SABC-immunohistochemistry. Primary antibodies: rabbit polyclonal antibodies against Bactrian camels IgG were from our laboratory (Veterinary pathology laboratory of college of veterinary medicine, Gansu Agricultural University, China).
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This rigorous cell preparation method and staining protocol was used throughout this study unless otherwise indicated.
Formalin-fixed, paraffin-embedded tumour samples were stained using a standard immunochemical method and staining was assessed on a four-point scale.
Paraffin sections (4 μm thick) were processed using routine methods and stained with HE.
Multiple tissues were taken for histopathology, processed by routine laboratory methods and stained by haematoxylin and eosin.
For histology, lung tissues were fixed in 10 % neutral buffered formalin and slides were processed by routine methods and stained with haematoxylin and eosin (HE).
The fixed tissue specimens were processed by standard methods and stained for Hematoxylin and Eosin (H & E) from Sigma-Aldrich using the protocol provided by the manufacturer.
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