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Transfection of the resultant plasmid into HEK293T SPLhi cells was performed via the Ca2PO4 precipitation method and stable clones were established by selection on 2 μg/ml puromycin for 3 weeks.
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New analytical tools, such as constant flux proxies, novel sediment grain size, and provenance methods, and stable isotopic analysis of plant wax biomolecules are reshaping our views on how and why African climate changed.
The second method is an implicit finite difference method and unconditionally stable.
In The numerical caschemedies the results obtained from the kinetic scheme are compresentedheree fisst order upwind and cexplicitschemethod
Comparisons with Nitsche's method and the stable Lagrange multiplier space proposed in Hautefeuille et al. (2012) [44], when they are feasible, highlight good performance of the approach.
Furthermore, applying the same method to load data from another four cities indicates that the proposed DLC method is adaptable and stable.
This method provides maximized and stable 64Cu labeling efficiency.
Numerical examples show that our proposed method is effective and stable.
Agrobacterium mediated transformation is traditional method for transient and stable genetic transformation because of several advantages (Hiei et al. 1994).
This paper presents a hybrid matrix method for simple and stable analysis of electromagnetic wave propagation in multilayered anisotropic materials.
Several numerical examples of two- and three-dimensional wave equations demonstrate that the present method is efficient and stable.
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