Sentence examples for method and sequencing from inspiring English sources

Besides the annotation method and sequencing time, some other factors also cause the different extent of errors.

AA, TP, MAM implemented the viral load quantification method and sequencing, participated in the data analysis and collaborated writing the manuscript.

The same sample preparation, DNA-extraction method and sequencing technique were applied as previously described for BGP_DF to ensure comparability of the metagenome datasets.

After pooling, dilution and emulsion PCR (emPCR), amplification products were sequenced on a Roche/454 GS Junior system, following the protocol described in the Roche emPCR Amplification Method and Sequencing Method Manual.

After that, the PCR product was purified by GenElute™ gel extraction kit (Sigma) method and sequencing of PCR product was done by Sanger sequencing method at 96°C for 1 min, at 96°C for 15 s, at 52°C for 30 s, and at 60°C for 4 min for 25 cycles with a hold at 4°C.

The 3' RACE method and sequencing were then used to identify the antisense transcript in mouse testis as well.

For the development of sequence characterized amplified region (SCAR) markers, a 747 bp amplicon was generated by the ISSR method and sequenced.

Libraries of 22 single cells were constructed using Smart-seq2 method and sequenced.

Sequence-PCR products were cleaned up using the ethanol/EDTA precipitation method and sequenced bi-directionally on an ABI 3130×l Genetic Analyzer (Applied Biosystems Inc., CA, USA).

This pool was amplified using the Lib-A method and sequenced on a 454 GS Junior system (Roche) at Landcare Research, Auckland.

After 35 cycles, the templates were purified by Ethanol/EDTA precipitation method and sequenced on ABI 3730xls Genetic Analyzer (Applied Biosystems) at Bioserve Biotechnologies, Pvt. Ltd.