Sentence examples for method and sequenced from inspiring English sources

For the development of sequence characterized amplified region (SCAR) markers, a 747 bp amplicon was generated by the ISSR method and sequenced.

Libraries of 22 single cells were constructed using Smart-seq2 method and sequenced.

Sequence-PCR products were cleaned up using the ethanol/EDTA precipitation method and sequenced bi-directionally on an ABI 3130×l Genetic Analyzer (Applied Biosystems Inc., CA, USA).

After 35 cycles, the templates were purified by Ethanol/EDTA precipitation method and sequenced on ABI 3730xls Genetic Analyzer (Applied Biosystems) at Bioserve Biotechnologies, Pvt. Ltd.

This pool was amplified using the Lib-A method and sequenced on a 454 GS Junior system (Roche) at Landcare Research, Auckland.

Sequencing libraries were generated from 200 ng mRNA using the cDNA Rapid Library preparation method and sequenced on the GS FLX Ti according to manufacturer's instructions (Roche, Burgess Hill, UK).

Besides the annotation method and sequencing time, some other factors also cause the different extent of errors.

AA, TP, MAM implemented the viral load quantification method and sequencing, participated in the data analysis and collaborated writing the manuscript.

The same sample preparation, DNA-extraction method and sequencing technique were applied as previously described for BGP_DF to ensure comparability of the metagenome datasets.

After pooling, dilution and emulsion PCR (emPCR), amplification products were sequenced on a Roche/454 GS Junior system, following the protocol described in the Roche emPCR Amplification Method and Sequencing Method Manual.

The PCR products were purified as described in Materials and Methods and sequenced (GeneWiz).