Sentence examples for method and resuspended from inspiring English sources

Specimens were digested and decontaminated using the Nalc-NaOH method, and resuspended in 2.0 mL of phosphate buffer.

Mononuclear cell suspension was obtained by the Ficoll-Hypaque density gradient method and resuspended in RPMI-1640 medium with 10% fetal calf serum.

To quantify DNA for real-time qPCR assays, genomic DNA was purified with the standard phenol-chloroform method and resuspended in 10 mM Tris-Cl buffer, pH 8.0, or diluted in distilled, sterile water.

For microarray and real-time PCR analysis, total RNA was extracted from harvested primary BRK cells and transformed cell lines by the Trizol method and resuspended in RNase and DNase-free H2O.

Briefly, spheroplasts were prepared from midlogarithmic phase K. pneumoniae ATCC 13883 cells using the lysozyme-EDTA method, and resuspended in 100 mM phosphate buffer (pH 7.5) containing 20% sucrose to an OD500 nm of ∼1.

For FAC recovery, we prepared ~0.3 mL of 10/mL protoplasts from A. nidulans FAC strains and FAC DNA was isolated by the common alkali lysis method, and resuspended in 10 μL of TE.

Genomic DNA was extracted from these samples following a standard phenol chloroform method [27] and resuspended in TE buffer (10 mM Tris, 0.1 mM EDTA, pH 8.3).

Total RNA was extracted by phenol-chloroform-isopropanol method, purified, and resuspended in 20 μL of RNase-free water.

Trophozoite-infected erythrocytes were harvested and enriched using the percoll/sorbitol method, washed, and resuspended at 0.1% hematocrit in osmotic lysis solutions containing either 280 mM sorbitol or 150 mM Gdm-Cl buffered with 20 mM HEPES, 0.1 mg/mL BSA, pH 7.4.

DNA is extracted by the phenol/chloroform method, ethanol-precipitated, and resuspended in water.

Genomic DNA was isolated using CTAB-NaCl extraction method [ 97] and resuspended in TE buffer (10 mM Tris pH 8, 1 mM EDTA pH 8).