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The method and reagents described in this paper significantly simplifies the construction of expression plasmids from multiple DNA fragments, including complex gene fusions, chimeric genes and polycistronic constructs.
However, the detection method and reagents are generally target-driven and the user must decide on what level (genetic versus phenotypic) the detection should be performed.
Here, using hydrothermal method and reagents of sodium citrate and polyethylene glycol (PEG), we synthesize the ZnO nano-crystals and find that they exhibit hierarchical flower-like architectures assembled by nanosheets.
Here, using simple hydrothermal method and reagents of polyethylene glycol (PEG-20000), we synthesize the ZnO crystals and find that they exhibit hierarchical flower-like architectures assembled by nanorods.
Individual investigators are allowed to specify the anatomic site, histopathology or concurrent disease, tissue preparation method, and reagents for use in tissue preparation in order to match pre-analytical variables to their research requirements.
The inter-batch variation increases to 27%, indicating that there is some variation in the quantity of this impurity across 20 batches of methamphetamine synthesized by the same chemist using the same equipment, method, and reagents.
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In current work, using the hydrothermal method and reagent of Cetyltrimethyl Ammonium Bromide (CTAB), we synthesize the 3D rose-like Cu2O nanoflowers with an average diameter of 200 250 nm, which exhibit rose-like nanoflowers assembled by numerous nanosheets.
Second, the values of HbA1c were not measured by high-performance liquid chromatography (HPLC) as used in the Diabetes Control and Complications Trial, although the method and reagent used to measure HbA1c in this study have since been NGSP-certified.
A number of methods and reagents have been reported recently in the literature for this purpose [5 14].
Soon I was one of the more prolific posters on the BioNet newsgroups, where I took up residence on the Methods and Reagents thread.
"Access to data, samples, methods, and reagents used to conduct research and analysis, as well as to the code used to analyze and process data and samples, is a fundamental requirement for transparency and reproducibility," the authors wrote, but "field sciences, if they even address such access, commonly do so by simply noting 'data and samples available upon request.'..
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