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In brief, FA-PLP nanoparticles were synthesized through a self-assembly nanoprecipitation method and radioactive FA-PLP (FA-PLP-131I) was prepared using the chloramine-T oxidation method [23, 24].
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Cell monolayers and growth medium were then extracted by the Folch method, and total radioactive phosphatidylcholine as well as disaturated phosphatidylcholine were determined by thin-layer chromatography and alumina gel fractionation of osmium tetroxide-reactive phospholipid, respectively.
Study design: We have tested the efficiency of PCR method and non-radioactive labeling of HSV DNA probe for detection of HSV from 30 clinical specimens (skin and mucous membrane swabs).
The conventional methods to study interfacial phenomena of biomolecules are often those of physical chemistry, such as optical, hydrodynamic, and radioactive methods.
The fluorescent and radioactive methods can be used separately or in combination to elucidate different aspects of lipid and lipoprotein biology, and potential roles in development.
In the previous manuscript version, the number of PCR cycles used was clearly stated in the relevant paragraph of the Methods section for both "MiSeq Library Preparation" and "Radioactive PCR".
The radioisotope method requires radioactive agents and therefore can only be performed at a limited number of hospitals because of the regulations for handling radioactive agents.
The Japan Society of Sentinel Node Navigation Surgery has conducted a prospective multicenter trial of sentinel node (SN) mapping by a dual tracer method using radioactive colloid and blue dye [ 19].
Postmortem lymphatic vascular casting [ 20- 22] and radioactive albumin clearance methods [ 17, 23- 25] have demonstrated the major contribution of these pathways in CSF drainage.
The decreased expression of classical hepatocyte/hepatoblast cellular function, namely synthesis of albumin and AFP, found in passaged rat fetal liver cells was confirmed, utilizing a very specific and sensitive method of the radioactive biosynthetic protein labeling and immunoprecipitation followed by SDS-PAGE analysis and autoradiography of the immunoprecipitates.
Group A (92 proteins) comprised substrates that were found only with the fluorescent detection method and were not found using the radioactive method; group B (88 proteins) were substrates that were found only with the radioactive method and not with the fluorescent approach; and group AB (26 proteins) were the substrates detected by both methods, with a significant overlap (P = 2.24 × 10).
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