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We also demonstrate the method and quantify its benefits on a nontrivial colloidal system of charged particles in the canonical ensemble.
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The antibacterial effects of these textiles were evaluated using ISO 20743 standard with the transfer method and quantified by the plate count method.
DNAs were extracted using the CTAB method and quantified using both a NanoDrop ND-1000 Spectrophotometer and agarose gel electrophoresis.
The ssDNA library of M299 was prepared from five µg DNA according to the GS FLX Titanium Library preparation protocol (Gel-cut method) and quantified and titrated similarly to the standard library by qPCR and test emulsions.
ARV strain S1133 was purified using the CsCl method and quantified as previously described [ 39].
The ribonucleic acid (RNA) was isolated by chloroform-isopropanol method and quantified.
The protein expression of p21/waf1 was assayed employing the western blot method and quantified using Image J densitometry software.
Total RNA from placenta samples was isolated using the Trizol method and quantified by the Nanodrop (ND1000 v3.5.2) spectrophotometer.
Total RNA from placental samples was isolated using Trizol method and quantified by using the Eppendorf biophotometer machine.
Proteins were visualized using the enhanced chemiluminescence (ECL) method and quantified by densitometry scanning with Quantity One software.
Immunoreactive bands were visualised with the ECL method and quantified using the Image J software (National Institute of Health (NIH), Bethesda, MD, USA).
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