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In addition, no post-PCR manipulations are required with this method, and quantification and calculation of the results are all automated.
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Extrapolation of the detailed results from this evaluation to other situations in terms of for example reconstruction methods and quantification methodology can thus not be made unreservedly.
In this paper both advanced calculation methods and quantification approaches for robustness are combined by a computational efficient calculation scheme which considers different levels of structural idealisation.
For molecular techniques, the available techniques include FISH-based techniques, PCR-based methods and quantification by quantitative PCR (qPCR) and RT-qPCR.
Information on tumor receptor status, as well as the available laboratory methods and quantification descriptions used to determine receptor status was collected by 20 centers.
Recently, progress has been made in better definition of factors influencing preanalytical sample processing and storage [ 15– 17], but variability between extraction methods and quantification approaches still presents major potential sources of experimental error [ 18].
Method calibration and quantification was performed by the pure reference standard (0.05 5 mg L−1).
Relative quantification and normalisation was done with the ΔΔCq method and transcript quantification was done twice to determine reproducibility.
The molecules were evaluated for their antifungal action against Candida albicans by cup plate method and ergosterol quantification method by UV spectroscopy.
Essential oil was extracted through hydro distillation method and the quantification was done by GC and GC MS instrument.
Expression values for TCF4 were calculated using the modified double delta Ct (ΔΔCt) method and absolute quantification and normalized to RPL13A [ 11].
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