Sentence examples for method and purified from inspiring English sources

AAV vectors were produced using adenovirus-free transfection method and purified by CsCl gradient centrifugation55,56.

All the mutations were generated using a PCR based method and purified using the same protocol as wild-type protein.

The seed oil was isolated by solvent-soaking method and purified by the alkali-refining technique.

Jeong et al. [110] synthesized SWCNTs by AD method and purified them by thermal and acidic treatments.

In fact, in our laboratory, several other recombinant proteins were produced by this auto-induction method and purified for biotechnological purposes, namely, structural protein-based polymers, human growth factors, serine proteases, hydrolases, esterases, lipocalines, antimicrobial peptides and plant transcription factors (personal communication, Casal, M).

Total RNA was extracted from animal caps with the TRIzol (Invitrogen) isopropanol method, and purified with the RNeasy Kit (Qiagen).

The vector was de-phosphorylated using shrimp alkaline phosphatase (Promega; Madison, WI) by standard methods and purified using QIAquick.

In addition, ELISA results commonly vary between studies conducted with different kits, extraction methods and purified standard proteins used.

Proteins were purified individually using cobalt beads (see methods) and purified COP was incubated with either purified wild type PLN or purified PLN RΔ14 (Figure 6E).

Genomic DNA was extracted from whole blood samples using standard salt-based methods and purified genomic DNA was aliquoted (10 ng·µL–1 concentration) into 96-well or 384-well plates.

35 Genomic DNA was extracted from whole blood samples using standard salt-based methods and purified genomic DNA was aliquoted (10 ng μL−1 concentration) into 96-well plates.