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Proteins were electro transferred to a PVDF membrane (Millipore Corporation) by a semidry transfer method and probed with indicated antibodies according to manufacturers instructions.
Proteins were transferred to nitrocellulose membranes using the semi-dry blotting method and probed with specific polyclonal rabbit anti-AMO or anti-pyruvate carboxylase (Pyc1; loading control) antibodies.
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The as-prepared catalysts are characterized using X-ray diffraction, transmission electron microscopy, standard electrochemical methods and probed for the electrochemical O2 reduction reaction (orr).
Tandem affinity purification of wild type SOAT and di-arginine mutated SOAT was then carried out (see methods) and probed for COP I binding.
AH was assessed by using the psychrometric method and probes temperature continuously monitored.
Normalisation was carried out using Invariant Set Normalization method and probe expression values were then calculated using the perfect match (PM -only model.
Otherwise, null values were replaced by the probe's average (row average method) and probes targeting the same gene were averaged.
In some experiments, PCR products were blotted onto nitrocellulose (Hybond N+, Amersham, Little Chalfont, Bucks, UK) by standard methods and membranes probed using P-labelled specific oligonucleotides.
It is necessary to adopt appropriate methods to fabricate and probe new multilayered structure films for further industrial application.
To this purpose, different methods and probes were comparatively used to infer, noninvasively, the relationship between the water content of stone materials and the reflection properties at microwave frequency of the material.
We feel that the most likely explanation is found in the different methods and probes used in each study to target this complex region.
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