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Stool samples were examined for helminths using the Kato-Katz method and polymerase chain reaction (PCR) [13] [15].
In this study, bacterial and fungal communities were investigated based on the culture-dependent method and polymerase chain reaction-denaturing gradient gel electrophoresis analysis.
In recent years, various chair-side microbial tests have been developed aiming at the detection of periodontal disease, including enzyme assay, DNA probe method, and polymerase chain reaction.
One miRNA TSS was identified as being the same by our method and polymerase II ChIP-chip, but not by 5′ rapid amplification of cDNA ends procedure.
Genotyping was conducted for CYP1A1 (Ile462Val; rs1048943), CYP1B1 (Leu432Val; rs1056836), COMT (Val158Met; rs4680), CYP17A1 (A1> A2; rs743572); C>P19A1 (C> T; rs10046) and CYP19A1 ((TTTA n repeats) polymorphisms and BRCA1 founder mutation using TaqMan allelic discrimination method and polymerase chain reaction based restriction fragment length polymorphism.
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Whether PSA is expressed in normal healthy breast is controversial [ 21], although Yu and colleagues [ 22], using quantitative methods and polymerase chain reaction, were able to demonstrate PSA in normal breast tissues in 33% of cases.
Each hour, enriched aliquots were compared using traditional culture methods and quantitative polymerase chain reaction (qPCR).
A cross comparison of DNA extraction methods and DNA polymerase used revealed that the DNA extracts (10 ng of DNA per PCR reaction) gained from the Analytikjena DNA extraction kit (AnaP) were suitable for the use of most polymerases, except for two enzymes.
Bacterial load was measured by colony-forming units (CFU) as per standard methods and quantitative polymerase chain reaction (qPCR) as previously described.
Employing blood from acute infected cows, the indirect ELISA response against p64 was contrasted with the microhematocrit centrifuge method and analyses by polymerase chain reaction (PCR) using specific primers targeting the inter-specific length variation of the internal transcribed spacer 1 region of the 18S ribosomal gene.
The extraction method and the quantitative polymerase chain reaction (qPCR) assay are previously described [ 10].
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