Sentence examples for method and pcr from inspiring English sources

Quantitative RT-PCR was analyzed via the ΔΔCT method, and PCR products were visualized by agarose gel electrophoresis.

PCR amplification of 20 ng genomic DNA was performed using a touchdown thermal cycling method and PCR products sequenced directly.

For the diagnosis of S. stercoralis, both the Baermann method and PCR showed low sensitivity (28.3% and 11.6%, respectively).

Serotyping of S. pneumoniae was done by Quellung method and PCR capsular typing was used for H. influenzae.

Fold changes were calculated using relative standard curve method and pcr controls included no template and no reverse transcriptase.

Agrobacterium tumefaciens strain EHA105 cells were transformed with the AtCuAO1-pC-TAP, AtCuAO2-pC-TAP or AtCuAO3-pC-TAP plasmids using the freeze-thaw method and PCR confirmed.

After cultivation, a varied set of isolates were detected using both phenotypic methods and PCR analysis of 16S rRNA (Randazzo et al. 2002).

Genotyping methods and PCR primers were provided by the suppliers, or are as outlined by Isaac, et al. [28].

At the Glu-D3 locus, allelic identification was problematic for the electrophoresis based methods and PCR.

Anthrax was confirmed using classical microbiological methods and PCR as described in Blackburn et al. [ 17].

All isolates were confirmed as N. gonorrhoeae by both phenotypic methods and PCR.