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The formulation was prepared by a high energy emulsification method and optimized by response surface methodology.
Glycyrrhiza polysaccharide liposome (GPSL) was prepared by reverse-phase evaporation method and optimized with response surface methodology.
The ClAlPc-loaded nanocapsules were prepared by the nanoprecipitation method and optimized by means of a 23 full factorial design.
The FDF was prepared using solvent casting method and optimized employing central composite design considering two independent variables film forming polymer (pullulan) and PEG 400.
In this study, the SE was prepared by the injection method and optimized by the single factor test and uniform design.
Formulations were prepared using solvent injection method and optimized by the Box-Behnken experimental design to get the desired particle size having maximized entrapment efficiency as well as percentage release.
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The mean Fm values between our previous method(15) and optimized method for any of the four C sources was not significant (p < 0.05) and our optimized method took only half a second to complete as our previous method.
Developing stringent analytical methods and optimized formulation dosage form is the goal of well-characterized biologics and drug development.
All PCRs were run by using standard methods and optimized annealing temperatures of 55°C through 65°C.
Therefore, improved delivery methods and optimized protocols for gene silencing in a tick's nymphal or egg stage would make RNAi more widely available to tick biologists and enhance future RNAi applications in tick-host-pathogen and high throughput tick genome research.
For the analysis of the data and selection of the optimal cutting parameters the Taguchi method and genetically optimized neural network systems (GONNs) were used.
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