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To improve the trackability of the mobile robot, we adopted a Bézier subdivision method and inserted a quintic Bézier segment into high-curvature areas.
The synthetic genes of CCR5, CCR3, CXCR4 and CX3CR1 were synthesized using a two-step assembly/amplification PCR method and inserted into two different kinds of expression systems.
For generation of transgenic lines we used the ɸC31 integrase method and inserted constructs in attP2 and attP40 docking sites (Groth et al., 2004; Markstein et al., 2008; Pfeiffer et al., 2010).
Plasmids from 97 individual clones harboring noisy yEGFP expression were extracted using a standard phenol chloroform extraction method and inserted fragments were sequenced using a primer hybridizing in the yEGFP gene 75 bp upstream of the start codon (supplementary table S7, Supplementary Material online).
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This observational study was designed to determine the importance of sterilization method and insert thickness as predictors of articular damage of cruciate-retaining polyethylene components used in total knee arthroplasty.
The whole DNA sequence was synthesized by a one-step total gene synthesis method, and then inserted in pUC18 vector.
The alloy components were built from cobalt-chromium in an initial powdered form using established digital technology methods and then inserted into a build of a resin base plate.
Experiments on animals have shown that Crispr/Cas9 works better than any previous gene-editing technique and offers a realistic alternative to gene therapy on human cells that does not involve viruses or other cumbersome methods of editing and inserting the corrected DNA sequences.
Cannulation was placed in the femoral vein of a rat and probes for measurement of the tissue oxygen partial pressure and microdialysis method were inserted into subcutaneous tissue of the abdominal wall.
A card that indicated the method given, date inserted and expiry date was given together with post insertion instructions on wound care.
This cassette, present in the Tn7-UAU1 transeeson (see Materials and methods), was inserted by in vitro transposition into each of the 39 target ORFs encoding putative GpiPs (Fig. 1a).
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