Different effects of EEDL on nitric oxide (NO) and prostaglandin (PG E2 secretion were investigated by Griess reagent method and enzyme-linked immunosorbent assay (ELISA) respectively.
However, each system has unique immobilized enzyme characteristics depending on factors such as enzyme source, support type, immobilization method, and enzyme-support interaction [ 29, 30].
The highest glucan, xylan and arabinose conversion were reached after 72 h of hydrolysis for all tested biomasses, pretreatment methods and enzyme mixes.
The highest glucan, xylan, mannose and arabinose conversions were reached after 72 h of hydrolysis for all the tested biomasses, pretreatment methods and enzyme mixes.
In this study, the choice of the proper disulfide bond formation was based on computational methods and enzyme functionality (active site position) which is called geometric-functional method.
The three participating laboratories used three different methods and enzymes for the indirect analysis (Table 4), with one method designed to replicate the CDC's methodology (Laboratory 2), one designed to replicate the NCTR/FDA's methodology (Laboratory 3), and a third method that included a higher concentration of enzyme (Laboratory 4).
Fasciola eggs and antibodies against Fasciola were detected by a quantitative sedimentation method and an Enzyme Linked Immunosorbent Assay, respectively.
Herein, poly N-isopropylacrylamide-co-N,N′-dimethylaminoethylmethacrylate) copoly N-isopropylacrylamide-co-N,N′-dimethylaminoethylmethacrylate one-stepoly N-isopropylacrylamide-co-N,N′-dimethylaminoethylmethacrylated in the films simultaneously, which were designated as poly N-isopropylacrylamide-co-N,N′-dimethylaminoethylmethacrylate
Glucose and insulin concentrations were measured using the glucose oxidase method and an enzyme immunoassay, respectively.
Plasma glucose and insulin were determined by the glucose oxidase method and the enzyme immunoassay, respectively, using commercial kits at the hospital laboratory.
