Sentence examples for method and digested from inspiring English sources

Plasmids containing the cloned fragments were isolated by the alkali method and digested with EcoRI.

DNA was isolated from overnight pure culture using the guanidine-EDTA-Sarkosyl method and digested with HindIII restriction enzyme.

The DNA was isolated using standard alkaline lysis method and digested with NotI (0.4 U/20 μl).

The BAC DNAs were isolated using a rapid alkaline lysis miniprep method and digested individually at 37°C for 3 h with 0.5 U Not I.

Closed circular DNA was extracted by the alkaline method and digested with PvuII to fragment all non-minicircle products of Cre recombination.

Proteins were extracted from intracellular and extracellular bacteria using the same method and digested for mass spectrometry as previously described [ 17].

For aCGH, high quality genomic DNA (0.5 to 3 µg) was isolated from frozen tumor specimens using standard methods and digested with restriction endonucleases AluI and RsaI according to the Agilent Oligonucleotide Array-based CGH for Genomic DNA Analysis Version 4.0 protocol.

RNA was extracted from varying amounts of baboon liver tissue by using the guanidinium-acid-phenol method (21 ) and digested with RNase-free DNase I (Fermentas, Waltham, MA, USA) to remove any contaminating DNA.

Total RNA was extracted using the hexadecyltrimethylammonium bromide (CTAB) method [ 22], and digested with RNase-free DNase I (Promega, Madison, USA) at 37 °C for 30 min.

DNA was isolated using the CTAB method and then digested with 100 g/mL DNase-free RNase for 1 h at 37 °C to eliminate RNA contamination.

After colony PCR screening, plasmids were purified from the positive clones by the alkaline lysis method and then digested with HindIII/BamhI.