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Finally, a simplified calculation method is used as the third method and checked with tests results.
Protein contents of the extracts were measured in triplicate by Bradford method and checked for quality and quantity by comparing pattern intensities in SDS-PAGE.
Overall survival was calculated using the Kaplan-Meier method and checked using the log-rank test.
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A variety of numerical tests have been performed to validate the method and check its performance.
Next we find out the initial basic feasible solution by any of the regular method and check its optimality.
Next we find out the initial basic feasible solution by any of the method and check its optimality.
More specifically, shale gas well casing design calculation method and check standard were modified, well structure and full bore hole trajectory design were optimized, drilling quality was improved, cement properties were optimized and cement sealing integrity during fracturing process was checked.
In order to test the validity of the method and check its accuracy, it was applied to a group of volunteers of ages ranging from 41 to 49 years old.
The identification of B3, encoding genes was based on the genome annotation (see material and methods), and checked by the use of the Pfam and INTERPRO programs (see material and methods).
To address the role of plasmid size/copy number variation and translational efficiency between the Basic and Delay circuits, we have constructed a Control Delay circuit (TC2G, see Material & Methods) and checked our induction kinetics (see Supporting Information S1 section B, Fig. S3).
Then we combined the results from both of the above methods, and checked these results based on public information.
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