Sentence examples for method and cell from inspiring English sources
For flow cytometry analysis, hearts were digested by the Langendorff method and cell- or cardiomyocyte-enriched fractions analyzed.
Two different methods containing cell free chemical assay (1-diphenyl-2-picrylhydrazyl method) and cell based antioxidant activity test were used to evaluate the antioxidant capacities.
The results told us that they should be equally paid attention to and minimized by the optimized preparation method and cell design.
Cell viability was assessed by the Trypan blue dye exclusion method, and cell proliferation was determined by the CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS; Promega, Madison, WI, USA).
Cell viability was quantified using the trypan blue exclusion method and cell proliferation was assessed by Bromodeoxyuridine (BrdU) incorporation assay (Cell Signaling, Danvers, MA, USA), according to the manufacturer's protocol (Suman et al, 2013b).
In the present study, we determined the incidence of apoptosis (detected using the TUNEL method) and cell proliferation (assessed using the Ki-67 antibody), and the balance between these cellular events in apparently normal epithelium, hyperplasia, atypical hyperplasia, and in situ and invasive carcinomas, as a model of disease progression.
The hepatocytes were isolated by collagenase perfusion method and cells were incubated with different concentration of carpet dust (100 5000 μg/106 cells) with various time (30 180 min) intervals.
Transient transfection of COS cells was performed using the calcium phosphate method, and cells were harvested 48 h later.
We then addressed the effect of the PARP inhibitor ABT-888 on cell survival and proliferation using the MTT method and cell-cycle analysis.
Single cells were prepared using the enzyme disaggregation method, and cells were incubated with fluorescein-isothiocyanate conjugated anti-mouse IgG to discriminate tumour cells from macrophages (Olive, 1989).
The tools, which comprise both computational methods and cell culture platforms, will measure toxicology from a network perspective, by examining varieties of interactions and processes within the cell.
