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Protein content was measured using a standard BCA Assay (Thermo Scientific, Waltham, MA, USA) and lysates were treated according to manufacturer's instructions, with slight method alteration in the detergent removal step.
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As a complementary method, alterations in the structure of actin cytoskeleton were visualized by fluorescent staining of F-actin.
Although the main oscillatory behaviors are detectable using these methods, alterations in gene expression such as changes of amplitudes and phase-shifts were not observed, especially because the altered light conditions were applied only for a short period of time.
In addition to this change of plan, there was an alteration in method so that material written for earlier volumes of the 11th edition could be altered after later volumes had been worked on, because all were published together over a period of less than two years.
In-situ-based technologies such as IHC and SISH/fluorescence ISH (FISH) are standardised methods for detecting genetic alteration in clinical practice, and data on the correlation between these methods would be critical to determine the guideline for MET evaluation of cancer patients.
We performed Scyl1 knock down in cultured cells using previously established methods and observed an alteration in Golgi morphology.
We currently aim to validate our findings in the human system and to improve our MALDI-MS proteomic methods to elucidate biochemical alteration in serum from young and aged human donors.
We made an alteration in this method of classification, adding one new class, the In-Cab Train Advisory Systems, reorganized the content of some classes, and extended the description of each class and their subsystems.
An objective and automated method for assessing alterations in gait and motor coordination in different animal models is important for proper gait analysis.
Similarly, a single unit showing no change in waveform or firing rate would result in near-zero values and so the subtractive method highlights only alterations in well-separated units' firing patterns or waveform.
The SPECT imaging method reflected the alterations in cardiac anatomy as well as the enlargement of the right ventricles in cardiomyopathic hearts found also by means of MR imaging (Fig. 9).
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