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This method also overcomes some of the limitations of SEM imaging in that the embryos never have to be dehydrated, can be kept at room temperature in fixative indefinitely, can be used on museum specimens, is non-destructive (unlike SEM), and also provides simultaneous tissue resolution below the differentiating skin.
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These normalization methods also overcome the variations in the FTIR spectra due to inconsistent sample thickness.
Here, two dimensional Hilbert transform based phase retrieval method is adopted in cellular phase imaging, it not only reserves more sample specifics compared to classical fast Fourier transform based method, but also overcomes disadvantages of traditional algorithm according to Hilbert transform which is a one dimensional processing causing phase ambiguities.
This method will also overcome the sample porosity or impurity problems commonly faced in the conventional indentation or deformation experiments.
Because these methods can also overcome many weaknesses of Dittrich's original investigations and more directly assess the validity of Dittrich's hypotheses, studies applying these methods on Dittrich's ASC questionnaires have long been overdue.
This method simplifies the measurement procedures and also overcomes the uneasiness in the conventionally used carotid-femoral PWV measurement.
Moreover, it outperforms the genetic algorithm in finding optimal solution and also overcomes the divergence problem caused by the Lagrangian method occurring at low demands.
These approaches are not only much faster than conventional alignment-based methods, but they also overcome some notorious difficulties in phylogenomics, such as finding ortholog genes (Ebersberger et al., 2009; Schreiber et al., 2009) or aligning large genomic sequences (Darling et al., 2010).
This method can effectively combine the mathematical methods and professional knowledge, and also overcome several drawbacks existing in the common decomposition methods, such as one-against-all, one-against-one and error-correcting output code.
Moreover, the limitation of detecting genetic interactions like A ⇔ B, which are prevalent in genetic networks [ 6], is also overcome with the proposed method.
Our approach can also overcome some of the limitations of traditional research methods in social science based on surveys or ethnographic observation [73].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com