In order to test the hypothesis that CBP and p300 exhibit different specificities, we employed a label-free, quantitative mass spectrometry-based method already established in our lab.
By developing methods to detect acetylation of histone H4, this research expands on the label-free mass spectrometry method already established in our lab, which allowed us to study each individual acetylated site on histone H3.
The indicators "Reduction of CO2 Emissions", "Reduction of CO Emissions" and "Reduction of Fuel Consumption" were calculated according to emission of polluting substances and consumption factors applied to traffic data, following methods already established in the literature [13].
Within each "main effect of time" image, functionally defined ROIs (also referred to as "clusters," below) were next identified using methods already established in the literature (Wheeler et al. 2005; Velanova et al. 2008).
Plasma inflammatory markers (including TNF-α and CRP), lipids (total, LDL- and HDL-cholesterol, and triglyceride), and satiety hormones (including GLP-1, PYY, ghrelin and leptin) will be measured using methods already established in the laboratory and described below.
This method is already established as a powerful tool in many fields such as marketing, political science, organizational behavior, epidemiology, sociology, and software development [15 17].
In the field of microarrays, it has traditionally been difficult to compare new methods with already established and published methods, as different strategies for preprocessing, summarizing and filtering make it almost impossible to work with the exact same data, even when the raw data is made available.
The mean tissue specificity for each of these groups was calculated using a method we developed based on Grubbs' test [ 23], and we validated this method using an already established method for ranking tissue specificity based on Shannon entropy [ 24].
Even though health risk methods are already established, majority are for existing plants assessment.
Within human risk assessment, dermal in vitro methods are already established and routinely used to assess the dermal uptake of test compounds after topical application.
Array-based methods are already established to screen hundreds of ORFs for possible Cpn human PPIs [ 17].
