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The determination of Ca, Cu, K, Mg, Na and Zn was performed using inductively coupled plasma emission spectrometry (ICP-AES, Jobin Yvon 238 emission-spectrometer, Instruments S.A., Division Jobin Yvon, Longjuemeau, France) with set-up and conditions according to the method accredited by SWEDAC (Swedish Board for Accreditation and Conformity Assessment).
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10 The method is accredited by the Danish Accreditation Authorities (DANAK) according to ISO 17025.
The analysis method is accredited by ISO 17025.
All the experiments, methods and facilities have been accredited by Ministry of Health: The Council for Experimentation on Animals, National Institute of Health Office of Laboratory Animal Welfaree and by Association for Assessment and Accreditation of Laboratory Animal Care AAALACC).
The method is based on the U.S. EPA 5030C (purge-and-trap for aqueous samples) method (U.S. EPA 2003) and is accredited by the Australian National Association of Testing Authorities.
These auditing organisations should be accredited by a national accreditation body.
The programs are accredited by the Global accreditation body AMBA.
Plasma CRP was assayed using automated methods on an Olympus AU2700 analyser (Olympus Diagnostica GmbH (Irish Branch), Lismeehan, Ireland), in the Department of Clinical Chemistry, Royal Infirmary of Edinburgh (fully accredited by Clinical Pathology Accreditation (UK) Ltd).
Anti-doping laboratories accredited by the WADA World Anti-Dopingg Agency) must have available methods capable of detecting synthetic steroids at concentrations below 10 ng mL−1 and, at the same time, be able to quantify endogenous steroids with accuracy.
All methods involving animals were approved by the Institutional Animal Research Committee (approval #07-0083) accreditedited by the American Association for Accreditation of Laboratory Animal Care.
Indirect phenotypic DST method was performed at the Reference TB laboratory at Pham Ngoc Thach Hospital, which is accredited by the WHO reference TB laboratory of Western Pacific region (Adelaide, Australia).
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