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Toluene and methanol were supplied by Fisher Scientific.

Sodium hydroxide, sodium hydrogen sulphite, chloroform, hexane, methanol were supplied by S.D. Fine Chemicals Ltd., Mumbai.

All solvents, including dimethylformamide (DMF), dichloromethane (DCM), chloroform, ethanol, and methanol, were supplied by Merck Co., Germany.

For the high-performance liquid chromatography [HPLC] analysis, acetonitrile and methanol were supplied from Fisher Thermo Fisher Scientificc, Fair Lawn, NJ, USA).

Other analytical grade chemicals, such as methylmethacrylate, 1,6-hexanedio1 diacrylate, styrene (ST), azobisisobutyronitrile (AIBN), isopropyl alcohol, polyphosphoric acid, surfynol 440, acetone, absolute ethanol, sodium hydroxide, and methanol, were supplied by Sinopharm Chemical Reagent Co. Ltd., China and were used without further purification.

HPLC-gradient-grade acetonitrile and methanol were supplied by Merck (Darmstadt, Germany).

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When the glucose was exhausted, methanol was supplied from 1 to 7 ml/l/h during the first 6 h.

Then methanol was supplied to maintain a relative dissolved oxygen (DO) content between 20 and 40% under the speed of 6 8 ml/l/h (Bai et al. 2010).

In the case that coke formation is selectively ignored (the formed aromatics are not further converted into the graphitic cokes), the alkylation step is first forced to be interrupted, as no methyl group from methanol is supplied.

In this first approach to obtain recombinant Bm86, Bd86 and Ba86 secreted to the culture medium, methanol was supplied at 1 ml·h-1·L of the initial fermentation volume for the first two hrs and then methanol supply was increased in 10% increments every 30 min to a rate of 3 ml·h-1·L.

Ammonium formate, methanol and acetonitrile were supplied by Fisher Scientific (Loughborough).

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