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Two hundred micrograms per milliliter stock solutions in methanol were prepared individually for each of them.
(d) Stock standard solutions—(1 mg/mL) stock solution of each of DRO, CAF, PCT and PAP in methanol were prepared.
Several of the anti-solvents mentioned above (acetone-water, ethanol-acetone, DMSO-water and methanol) were prepared, and the selection of an anti-solvent for efficient saccharification was performed.
The α-glucosidase was assayed using a method modified by Apostolidis et al[ 20], where a 50% ethanolic extract of O. stameinus (62.5, 31.25, 15.6, 7.8, 3.9, and 1.95 mg/ml in dimethylsulphoxide:distilled water (1 1) solution) and sinensetin (2.5, 1.25, 0.625, and 0.3125 mg/ml in methanol) were prepared.
Several eluents with increasing/decreasing concentration ratio (ranging from 1 9 to 9 1) of chloroform to methanol were prepared to determine the best solvent system that separates components of the mixture in the extract; the 9 1 chloroform-methanol was used as solvent system in column chromatography.
The inhibition of α-amylase was determined using an assay modified from the Worthington Enzyme Manual [ 21], where a 50% ethanolic extract of O. stameinus (62.5, 31.25, 15.6, 7.8, 3.9, and 1.95 mg/ml in dimethylsulphoxide:distilled water (1 1) solution) and sinensetin (2.5, 1.25, 0.625, and 0.3125 mg/ml in methanol) were prepared.
Similar(50)
The derivatised TEA standard solution (1.91 µg ml−1, 5.071 µmol l−1 in methanol) was prepared by mixing 1 ml of the 10 µg ml−1 methanolic TEA solution with 1 ml DNPH solution.
For this purpose 0.1 mM solution of DPPH in methanol was prepared.
Stock solution containing 100 mg of glycyrrhetic acid in 100 mL of methanol was prepared.
A solution of 0.5 M H2SO4 in 2 M methanol was prepared as an electrolyte.
0.1 M solution of Zn(CH3COO 2·2H2O in 50 ml methanol was prepared.
More suggestions(15)
methanol was prepared
methanol were chosen
methanol were injected
methanol were generated
methanol were compared
methanol were used
methanol were added
methanol were procured
methanol were received
methanol were applied
methanol were evaluated
methanol were loaded
methanol were tested
methanol were investigated
methanol were supplied
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