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Exact(9)
Briefly, equal volumes of 1 M NH4Cl in H2O and 2 mM CFZ in methanol were mixed with the addition of a surfactant (up to 2% (v/v) TritonTM X-100, Sigma-Aldrich, Catalogue No. X100) and incubated at room temperature for 48 hours.
Briefly, equal volumes of protein extract and methanol were mixed together with one quarter of volume of chloroform.
A 3.2 ml of KOH (aq., 5 N) and 3 ml of methanol were mixed together in a 25 ml flask.
Briefly, 9 volumes of 4.4 mM butylated hydroxytoluene in HPLC-grade methanol were mixed with 1 volume of 1 mM xylenol orange and 2.56 mM ammonium ferrous sulfate in 0.25 M H2SO4 to give the "working" FOX reagent.
For the incorporation of BTA-Cy3 into the polymers, appropriate volumes of BTA-3OH and BTA-Cy3 from stock solutions in methanol were mixed and dried after which the protocol described above was continued.
The fifth generation (G5.0) PAMAM dendrimers in methanol were mixed with N-hydroxysuccinimidyl 3-mercaptopropanoate in 1 1 mixed solution of tetrahydrofuran and methanol and vortexed for 1 h at room temperature.
Similar(51)
700 µL of each concentration of the standard solution of ascorbic acid and test compounds in methanol was mixed with the same volume of methanolic solution of 700 µmol/L DPPH.
The total flavonoids content was determined using the Dowd method [ 14]. 5 mL of 2% aluminium trichloride (AlCl3) in methanol was mixed with the same volume of the methanolic extract solution (0.4 mg/mL).
5 ml of DPPH solution in 90%% methanol was mixed with an equal volume of the extract and ascorbic acid.
A volume of 0.100 mL of each astaxanthin sample, dissolved in methanol was mixed with 2 mL of 2%% (w/v) sodium bicarbonate and was incubated at room temperature for 2 min [19].
Microcystin LR (5.0 mg in 100 µl methanol) was mixed with DMSO (200 µl), 2-dimethylaminoethanethiol hydrochloride (7.0 mg in 200 µl H2O), and 3 µl of 500 mM EDTA (pH 8.0).
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