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The fractions (n- hexane, dichloromethane, ethylacetate and methanol) were concentrated to dryness using rotary evaporator and weighed.
The gular patch tissue samples stored in methanol were concentrated and directly analysed by gas chromatography mass spectrometry (GC-MS) methods.
The eluates (methanol) were concentrated and analyzed using HPLC/tandem MS (Olsson et al. 2004) with both quantification and confirmation ions monitored.
Similar(57)
The retained material eluted with 60% methanol was concentrated by using an evaporator and loaded onto an immunoaffinity column.
The methanol extracts were concentrated with rotary evaporator.
In brief, the methanol extracts were concentrated and lyophilized with a rotary evaporator and partitioned between water and n-hexane.
The hexane and methanol extracts were concentrated under reduced pressure at 45°C while the methylene chloride extract was concentrated at 50°C without the pressure reduction.
The filtrates from acetone, methanol and ethanol were concentrated under reduced pressure 40°C using rotary evaporator (Cole Parmer SB 1100, Shangai, China).
The methanol and chloroform extracts were concentrated in a rotary evaporator (Buchi type TRE121, Switzerland) at a temperature of 45 °C; whereas the aqueous extract was freeze-dried using centrifugal freeze dryer (Model 5 PS, Christ, England).
The crushed material of T. decurrens (12 g) was repeatedly extracted with methanol and the combined extracts were concentrated under reduced pressure.
Eluted proteins were concentrated by methanol precipitation and analyzed by SDS-PAGE.
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