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Exact(8)
Three rinse steps with methanol were applied under the same operation.
In short, serial dilutions (each dilution in triplicate) of pure glycosphingolipids in methanol were applied in microtiter wells (Falcon 3911, Becton Dickinson Labware, Oxnard, CA).
Derivatised steroids reconstituted in 1 mL of 10% methanol were applied to the column.
Additionally, dimethyl yellow and thymolphthalein dissolved in methanol were applied, but before used, the chromatographic plate was sprayed with 5% NaOH.
A 20 μl reference standard gymnemic acid (1 μg/μl stock solution) of 92% purity prepared in methanol were applied to the TLC plate.
Briefly, quantities of Cu II -ZBM (0–500 μg) in methanol were applied to 7 mm paper disks (Whatman) and dried.
Similar(52)
A 1000-fold sample dilution with methanol was applied to avoid column overload and contamination.
After dewaxing and rehydrating, an endogenous peroxidase block (0.3% H2O2 in methanol) was applied for 30 minutes.
DON- or ZEA-containing methanol was applied separately into the culture medium to achieve a final concentration of 5 and 10 ppm, respectively.
Next, ∼150 nL of acidified methanol was applied to the cell-containing spot; the small sample size allows for successful and quick analyte extraction.
The chemostats were started at D = 0.1 h−1 and a mixed feed of glucose and methanol was applied according to the following regime between the different steady states: glucose/methanol: 10 mM/15 mM; 5 mM/15 mM, and 5/30 mM.
More suggestions(15)
methanol were injected
methanol were prepared
methanol were generated
methanol was applied
methanol were compared
methanol were used
methanol were added
methanol were procured
methanol were received
methanol were evaluated
methanol were loaded
methanol were tested
methanol were investigated
methanol were supplied
methanol were introduced
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