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Exact(56)
After incubation for 1 min at room temperature, 80 230 μL sample solutions (4 mg/mL in methanol) were added.
Three volumes of methanol were added to the supernatant, and the mixture was kept at 4 °C overnight.
40 µL of methanol were added to the vial and they were then evaporated under a nitrogen stream.
Ten grams of the lyophilized samples were weighed and 100 mL of methanol were added to the samples in a 200 mL glass flask.
About 5 ml (10 ml) methanol were added to 10 ml (20 ml) RUSITEC sample and afterwards the pH-value was adjusted to 4.5 5.5.
Acetone and methanol were added separately into a glass container by heating only the acetone container onto a hot plate to warm up (not exceeding 55°C).
For each sample, an accurately weighed 0.5 g of the dried powder was introduced into a 50 mL calibrated flask and 20 mL of 70% methanol were added.
Similar(4)
Briefly, 2 μl of H6-acetic anhydride or D6-acetic anhydride [50% (vol/vol) in methanol] were added to 100 μl of peptide mixture in 50% methanol/water (vol/vol).
When fully dissolved, 100 μL of each sample were transferred to a 3.5 mL glass vial; the remaining 300 μL were dried overnight in a fume hood and frozen at −20 °C and 650 μL of 1 1 chloroform:methanol, D25-tridecanoic acid as internal standard to a final concentration of 21 μM, and 125 μL 10%% BF3/methanol were added to each sample.
The upper phenolic phase was transferred to a 50 mL tube, and then five volumes of 100 mM ammonium acetate/methanol were added.
The pods of single seeds were removed and then crushed in a plastic tube with screw cap and 300 μl of hexane and 40 μl of KOH (5 M /methanol were added, followed by vortexing and heating samples at 60°C for 6 min.
More suggestions(15)
meoh were added
formaldehyde were added
methanol were included
methanol were concentrated
methanol were chosen
methanol were injected
methanol were prepared
methanol were generated
methanol were compared
methanol were used
methanol were procured
methanol were received
methanol were applied
methanol were evaluated
methanol were loaded
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