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Exact(43)
The mixture was cooled to room temperature, acidified with 2 M HCl and the methanol was removed in vacuo.
Induction with methanol resulted in the rapid secretion of IFNα2b, which reached maximal productivity after only 3 h and remained constant during the production period, and then rapidly decreased after methanol was removed from the media.
Dynamic image analysis confirmed that minimum attrition of COA was achieved when (a) the majority of the methanol was removed during pressure filtration at 0.5 bar N2 and (b) intermittent agitation was applied during the vacuum drying stage.
The excessive methanol was removed using rotary evaporator.
Subsequently, methanol was removed under vacuum at room temperature in a Schlenk-line.
The methanol was removed from the product using a rotary evaporator at 313 K, followed by cooling to room temperature.
Similar(17)
After the methanol solvent was removed at 70°C, the sample residue was dissolved with 2 mL distilled water, followed by the addition of 1 mL concentrated hydrochloric acid.
After the methanol solvent was removed at 80°C, 0.1 mL vanillin-acetic acid solution (5 mg/mL) and 0.4 mL perchloric acid were added to the sample residue.
After incubation, 105 cells were labeled with FITC-conjugated c-kit antibody, fixed in PBS 1% formaldehyde-free methanol that was removed before treatment with 1 ml of PI/Triton X100 (20 µg/ml/0.1%)+DNase-free RNase (0.2 µg/ml) for 30 min at room temperature.
Extracts were eluted into methanol, which was removed under a stream of nitrogen.
The methanol extract was removed and the pellet was washed twice with distilled water.
More suggestions(16)
mark was removed
formaldehyde was removed
methanol was shown
methanol was sampled
methanol was procured
methanol was continued
methanol was adopted
methanol was observed
methanol was chosen
methanol was prepared
methanol was evaporated
methanol was added
methanol was used
methanol was conducted
methanol was employed
methanol was sonicated
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