However, the minimum boiling azeotrope, which has methyl acetate and methanol (0.82 and 0.18 wt fractions) as its constituents is being removed continuously, and the conversion is limited by methanol available for reaction when equimolar quantities of acetic acid and methanol are employed.
AC-18 reverse phase column eluted with a mobile phase (pH 4, 0.1 M sodium acetate, 0.1 M citric acid, 0.7 mM sodium octylsulphate and 0.57 mM EDTA containing 10% methanol, v/v), was employed.
For the pyridine and pyridine- d3 images, methanol- d4 was employed as the solvent and the acquisition parameters used were echo time (TE) 7.5 ms, field of view (FOV) 40 mm × 40 mm, slice thickness 2 mm, and acquisition matrix 64×6464×64
For the fuel cell, methanol and oxygen was employed as an anode and cathode, respectively.
Methanol or ethanol was employed depending on the targeted compounds [9].
Five millimole per liter ammonium formate with 0.01 % formic acid in water (solvent A) and methanol (solvent B) was employed as mobile phase.
As the maximum specific growth rate on pure methanol as a carbon source would be significantly lower, and intracellular carbon fluxes could not be analyzed on methanol alone, a mixed feed strategy applying glycerol-methanol co-feeding was employed.
A methanol-glycerol mix of 8.5 g/L methanol and 49.0 g/L glycerol was employed based on experiments with P. pastoris proving that total methanol utilization and full induction of the methylotrophic pathway were realized under these conditions.
Diafiltration (DF) was employed using methanol as the solvent needed in the next synthesis step to drastically reduce the concentration of ethyl acetate used as the solvent in the previous synthesis step.
