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Elution with methanol was continued for a further 6 min.
Then feeding with methanol was continued for a further period of 90 h.
Similar(58)
After discontinuation of the dialysis, the methanol level remained between 0.05 and 1.6 (mean 0.59) mg/dl, and infusion of 10% ethyl alcohol was continued until methanol level below 0.3 mg/dl was detected.
To N-functionalized cyclam (1.0 eq) was added copper II) chloride or zinc II) chloride solution in methanol (73.4 mM, 1.0 eq) and stirring was continued at rt for 10 min. Methanol was evaporated in vacuo and HEPES buffer (10 mM containing 100 mM NaCl) was added to give a final ligand concentration of 1000 µM.
The solution was cooled to 0 °C, methanol (3 mL) was added, and stirring was continued for 30 min. The solution was coevaporated with toluene.
Stirring was continued overnight, and when TLC showed complete reaction, methanol was added and the solution was evaporated in vacuo.
Methanol (1% v/v) was added after every 24 h to the culture and induction was continued for 4 days.
The work is continuing.
Methanol is contained in aspartame and is metabolized into deadly formaldehyde within the brain.
Methanol was added as organic phase.
10µl methanol was used as solvent control.
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