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Exact(6)
The standard solution of DPhT was prepared by dissolving DBTCl2 in 0.005 mol L−1 HCl in methanol, to prevent self-disproportionation.
The samples of saturated solutions were collected after filtration through a 0.22 μm filter (cellulose acetate filter, Sartorius, Goettingen, Germany) and diluted appropriately with methanol to prevent the crystallization of the solute.
The samples were spiked before extraction with the internal standard mixture, 100 μl 0.15 M EDTA and 10 μl BHT (2 mg/ml in methanol) to prevent oxidation of analytes.
Four-micron sections (4 μm) of representative blocks from each case were deparaffinized, rehydrated, and treated with 0.3% H2O2 for 5 min in methanol to prevent endogenous peroxidase activity and were immunostained by the peroxidase method (Envision System, DAKO, Carpinteria, CA, USA).
Four-micron sections (4 μm) of representative blocks from each case were deparaffinized, rehydrated, and treated with 0.3% H2O2 for 5 min in methanol to prevent endogenous peroxidase activity and were immunostained by the peroxidase method (Envision System, DAKO, Carpinteria, California, USA) according to the manufacturer's recommendations.
After initial incubation for 5 min in methanol to prevent the precipitation of hydrophobic peptides the membrane was rinsed with 1xTBS (50 mM TRIS, 137 mM NaCl, 2.7 mM KCl, pH adjusted to 8.0 with HCl) and blocked in 2% (w/v) skim milk powder in 1xTBS (2% M-TBS) for 1 h at room temperature on a rocker.
Similar(54)
The solution was filtrated with tempered syringe filters (0.45-μm PET) in tempered vials four times, and each was diluted 1 1 with methanol immediately to prevent precipitation.
4a Prior to reduction of the 4-azido group of 21, the acetyl groups were removed by transesterification in methanol in order to prevent formation of a 4′-acetamido product.
Immediate quenching and cooling with methanol to -30°C prevented the metabolites from further reactions.
The robots were expected to insert the sensors through a line used to inject methanol, an antifreeze meant to prevent the buildup of icy slush, into the containment cap over the ruptured pipe, said a BP spokesman, David Nicholas.
The additional catalyst layer formed an assistant cell with the cathode to prevent methanol crossover.
Related(20)
thiourea to prevent
methanol to inhibit
mercury to prevent
mark to prevent
substance to prevent
compound to prevent
formaldehyde to prevent
methanol to remove
methanol to permeabilize
methanol to produce
methanol to quench
methanol to keep
methanol to give
methanol to extract
methanol to run
methanol to lauric
methanol to block
methanol to seep
methanol to go
methanol to prepare
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