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The fuel (methanol) is kept constant for all the cases.
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Five different catalyst loadings (i.e., 2.0, 5.0, 10.0, 15.0 and 20.0 % g catalyst/g Sal oil) were considered in the present study and molar ratio of Sal oil to methanol was kept constant at 1 12 for all the catalyst loading experiments.
The concentration of methanol was kept stable by monitoring the dissolved oxygen (OD) content and maintaining it at greater than 20%.
Accumulation of methanol leads to cytotoxic effects (Guarna et al. 1997) and induction of the promoter is optimal when the methanol level is kept within growth limiting rates.
The fuel (methanol) flow rate is kept constant for all the cases.
In brief, 50 g of the methanolic extract was dissolved in 150 ml methanol and was kept at 2 8°C for overnight.
After sonication for 1 min, the methanol solution was kept at 4 °C for ca. 20 h.
Some of these reactions - a total of 13 - appeared to be relevant for growth on methanol and were kept in the methylotrophic network.
However, due to the small window in which the methanol concentration must be kept for an optimal process, pulse-based methanol addition for optimization of processes in shake flasks seems not to be the best way to provide data for the development of a fermentation process.
This suggests that the methanol concentration should be kept higher than a threshold in an ionic liquid based fuel cell.
During drug exposures, the concentrations of methanol and DMSO were kept below 1% and 0.001% respectively in treated and control wells.
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