Sentence examples for methanol for at from inspiring English sources

Both adherent and floating cells were collected and fixed in 100% methanol for at least 1 h at −20°C.

For immunofluorescence microscopy, the harvested cells were washed in PBS buffer and fixed in cold methanol for at least 20 minutes at −20°C.

Cells were harvested, washed in PBS and resuspended in 500 ml PBS and fixed by the addition of 2 ml of cold 90% methanol for at least 2 h.

Dissected gonads were fixed using 3% formaldehyde, 0.1 KpH4 (pH 7.2), for 10 min. The gonads were then postfixed in −20° 100% methanol for at least 10 min. Fixed gonads were incubated with 100 ng/mL of 4,6-diamidino-2-phenylindole hydrochloride (DAPI) in 1× phosphate-buffered saline for 5 min to visualize nuclear morphology.

After this period, the HEp-2 cell monolayers were washed with phosphate-buffered saline (PBS) (pH 7.2) and the attached cells were prefixed with 100% methanol for at least 20 min at 0°C or kept at this temperature until use.

For immunofluorescence, cells were fixed for 10 min either with 4% paraformaldehyde at room temperature or with a 1 : 1 mixture of acetone and methanol for at −20 °C (for γ-tubulin), and stained with mouse anti- α-tubulin (B-5-1-2, 1 : 2000), mouse anti- γ-tubulin (GTU-88, 1 : 1000; Sigma-Aldrich), and rabbit anti-phospho-histone H3 (06-570, 1 : 200; Millipore, Billerica, MA, USA).

Pretreatment steps were performed for some antibodies as follows: 2N HCl for 30 min at 25°C for BrdU and 100% methanol for 7 min at −20°C for MAG.

Cells were then fixed in 100% methanol for 10 min at -20°C, air dried, and stored at -80°C until use.

Oocytes were exposed to 2 M dimethyl sulphoxide (DMSO), propylene glycol (PG), and methanol for 40 min at 22 °C.

Sections were dehydrated in methanol/H2O series: 20%, 40%, 60 %, 80 %, 100 1 h each and incubated in 66% DCM/33% methanol for 3 h at RT. Then they were incubated in 100% DCM for 15 mins twice to wash meachnol.

The sensor was immersed in 1 mg ml−1 solution of 1-pyrenebutanoic acid, succinimidyl ester (Sigma-Aldrich, USA) in methanol for 2 h at room temperature and further cleaned with methanol.