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All substrates were treated in methanol before the deposition process.
The resulting solid was dissolved in methanol, before the addition of aqueous NaClO4.
The resulting mass was then dissolved in methanol, before the addition of an aqueous solution of 1 g of NaClO4.
The chloroform fraction was concentrated to dryness and dissolved in 10% water in methanol before the extraction with hexane.
Endogenous peroxidase was blocked with 3%H2O22 in methanol before the incubation with polyclonal rabbit-anti-TRIB3 (Novus Biologicals, Cambridge, UK) or rabbit-anti-pimonidazole.
In this study, 500 mg of ground RS were transferred in 100 mL glass syringes (Haberle Labortechnik, Germany) followed by the addition of 200 μL of methanol (control) while in the treatments, 10 mg (treatment 1) and 20 mg (treatment 2) of dry FRSE were dissolved in the 200 μL methanol before the latter was added in the glass syringe contain 500 mg RS.
Similar(54)
A rehydration process was then performed with descending grades of methanol before removing the coverslip and counterstaining in Mayer's hematoxylin for 1 min. The process of dehydration and mounting was then repeated.
Each 250 mL of filtered sewage sample were spiked with 100 μL of a surrogate standard solution prepared in methanol (bupropion-d9 / 10,11-dihydrocarbamazepine 10,11-dihydrocarbamazepine 2.5 mg/Lf methandl before lowering the pH to addition with 100 μL of phosphoric acid (85%).
The UV spectra of PdCl4 2− and 4-VP, and ANA and AAA in methanol before and after the interaction are shown in Fig. 1.
30 Briefly, the sections were washed with PBS, subjected to antigen retrieval by heating in a microwave in 0.01 M sodium citrate buffer (pH 6.0) for 15 min, and exposed to 3%H2O22 in methanol before staining with the primary antibody.
Preparations were rinsed six times with PBS to remove the methanol before use [ 26].
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