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The product was repeatedly washed with methanol and subsequently dried in an oven at 60°C until reaching a constant weight.
Also it may be understood that the glycerol would largely dissolve in excessive methanol and subsequently inhibit the reaction of methanol to the reactants and catalyst, thus interfering with the separation of glycerin, which in turn lowers the biodiesel yield by shifting the equilibrium in the reverse direction [24].
For staining of CD-3 and eNOS expressing cells, slides were heated in 10 mM Na-citrate (pH 6.0) for 20 min. Endogenous peroxidase activity was blocked with 0.3% H2O2 in methanol and subsequently, slides were blocked with either normal goat serum (eNOS and MPO) or bovine serum albumin (CD-3) for 30 min at room temperature.
Blood smears were fixed in methanol and subsequently stained with Wright-Giemsa.
It was dissolved in dichloromethane and precipitated in methanol and subsequently dried in vacuum oven for 24 h.
The SPE column was washed with 1 mL of methanol and subsequently with 1 mL of water.
Similar(46)
Standard solution of diuron in water was prepared by transferring small proportion of the methanol solution and subsequently diluting the same with DI water.
Samples were then given a final rinse with a 70% (v/v) methanol solution and subsequently air-dried for 24 hours in a fume hood.
Coverslips with the cell monolayers were harvested, rinsed with phosphate buffered saline (PBS), fixed with 70% cold methanol, stored and subsequently immunostained following protocols similar to that described in detail previously [ 18, 19].
FeCl3 (0.21 mol) was dissolved in 60 ml of methanol; then, CTAB (if any) was dissolved in 40 ml of methanol and was subsequently added to the solution of FeCl3.
The metabolites in the filtered medium were extracted by using iced 50% methanol and were subsequently dried by a speedvac.
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