Sentence examples for methanol and finally from inspiring English sources

The replicas were then washed in chloroform, methanol, and finally, distilled water.

Then, the reaction mixture was refluxed for 2-6 h where the solid complexes were precipitated, filtered off, washed with methanol and finally diethyl ether and then dried in vacuo.

At the end of polymerization, the produced polymer was filtered on a filter paper and washed with 1 M HCl, acetone, methanol and finally with distilled water to remove any impurities.

The mixture was filtered through a filter paper (Whatman No. 41 grade) and washed thoroughly, first with double distilled water, then with methanol and finally with double distilled water until pH 7.0 ± 0.1 was attained, and finally air-dried and stored in a vacuum desiccator for future use (Okieimen and Okundaye 1989; Onwu and Ogah 2010; Srivastava et al. 2012).

For the samples C and D, 50 nm of Si was subsequently etched away using ICP dry-etching with an ambient gas mixture of SF6 O2 (20:4%) at a chamber pressure of 30 mTorr and RF power of 30 W. Followed by the dry-etching, samples were cleaned in a boiled acetone at 120°C for 5 min and in a methanol and finally rinsed in DI water (boiling temperature of acetone is 56°C).

Medium was changed every 3 days and after 10 days clones were washed with PBS and fixed in 10% formaldehyde (Sigma-Aldrich) for 15 min. Plates were then washed once with water and stained for 5 min in 0.5% crystal violet (Fluka, Sigma-Aldrich) in 20% methanol and finally washed with water to remove background staining.

Samples were rehydrated immediately after fixation in 60% methanol in distilled water (once), then 30% methanol (once), and finally PBT solution for five washes.

The suspension was discarded and the cell pellet was washed twice using PBS, stained with X-gal, fixed in methanol solution, and finally observed for staining status under a light microscope to determine the rate of infection.

HEK293a Shh+ cells were labelled with probe 2 or vehicle control (DMSO), fixed with paraformaldehyde, permeabilised with methanol (MeOH), and finally subjected to CuAAC ligation to azido-Alexa488 followed by confocal fluorescence microscopy.

An Oasis HLB cartridge was pre-conditioned with methanol (6 mL), 10% methanol (6 mL) and finally 70% methanol (4 mL).

The F-MWNTs were Soxhlet-extracted with water for 72 h to remove PPA, unreacted p-amino benzoic acid and methanol, and were finally dried under a reduced pressure for 3 days at 100 °C.