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A 250 microliter aliquot of EBC was gas-standardized by bubbling with Argon for 8 minutes at 350 ml/min prior to pH measurement, which was performed with an Orion pH glass combo microelectrode attached to an Orion 520 A meter as previously reported[ 5].
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The boundary between 'shallow water' and 'deep sea' for stylasterid corals was defined at a depth of 50 meters, as previously adopted for corals in general [16].
The WDR is a ratio of the percentage of weight carried on each hind leg in which the weight-bearing forces of both hind limbs were measured with an incapacitance meter (UGO-BASIL Biological Research Apreviously as previously described by Hwang et al. [ 15].
Rinse and dry as previously explained.
The muscle strength in the forelimbs was measured with a grip meter (TSE Bad HamburgGermanyasy) as previously described [15].
Both habitual and maximal gait speed were measured over a straight path of six meters (as performed previously in other trials (Gotlin et al 2000; Menz and Lord 2001), with a preceding acceleration lane of two meters, and a two meter deceleration lane following the six meter path.
Toronto Parking Authority removed some parking meters as well.
6-meter walk-time required participants to walk 6 meters as quickly as possible to do safely.
Prior to testing (training or trial) animals were acclimatized to the testing room for 30 min. Forelimb motor dysfunction was assessed using a grip strength meter (GSM TSE Systemss, Bad Homburg, GER) as previously described [11], [33].
The barrier was recorded with an EVOM meter every 24 h as previously described [ 24].
Total activity and time spent in the outer zone were monitored in a novel, open-field environment (42 × 42 cm; 30 min) by an Opto-Max animal activity meter (Columbus Instruments, Columbus, OH), as previously described (22, 23).
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