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The metagenomic and metatranscriptomic coverage of the methane metabolism pathway is shown in Fig. 1.
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With a four fold increase in cellular abundance, mitigated by an increase in dominance (Table 6), a decrease in metagenomic coverage is expected; conversely the sustained metatranscriptomic coverage is likely to be a direct result of the increased dominance of specific taxa, and hence specific transcripts.
However, the percentage of annotated transcripts was highest in the night compared to day samples from January and April (although this was not observed in August), which inversely correlated with a reduction in metatranscriptomic coverage (R = −0.79, p<0.05; Fig. S4).
As expected, during January and April, metatranscriptomic coverage was greatest during the day when diversity was lowest (Fig. 1).
For this study, coverage of pORFs by SEED annotation ranged from 20 46% of each metagenomic dataset and 11 35% of the metatranscriptomic datasets (Table 1).
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