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The tumor diameters in metastatic cases were significantly larger (Fisher's exact test: p < 0.0001).
The primary tumor diameters in the metastatic cases were 15.0, 120.5 20.5, 13.0, and 5.0 cm, respectively.
Comparing metastatic with nonmetastatic cases, the tumor diameters in metastatic cases were significantly larger (Fisher's exact test: p < 0.0001) (Table 2).
Notably, no significant differences between non-metastatic and metastatic cases were found concerning this specific lymphovascular histoarchitecture.
Our study is limited by its single-centre, retrospective design and by the relatively small sample size utilised, where only 10 metastatic cases were represented.
Increased serum levels of IL-6 were previously reported in progressive recurrent breast cancer patients [ 44], especially in the presence of metastasis [ 26], conversely no metastatic cases were enrolled in our cohort.
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Notably, in none of the investigated factors could significant differences between lymphogenously and haematogenously metastatic cases be assessed.
The distribution of genotypes across metastatic cases was as follows: three were sporadic (negative for all the mutations tested), two patients tested positive for mutations in SDH-B, one for SDH-D, one for VHL and one for NF-1.
Despite many advances in neuroblastoma research, 60% of neuroblastoma, which are essentially metastatic cases, are associated with poor clinical outcome due to the lack of effectiveness of current therapeutic strategies.
In our evaluation the AUC for ERG in discriminating localised prostate cancer from metastatic cases was 0.61, performing more poorly than as a discriminator of localised prostate cancer from benign tissue samples.
Again, a similar picture is obtained (Fig. 2): two clusters with a clearly different content of metastatic cases are obtained and the "good prognosis" cluster contains six misclassified cases, among which the five samples that are most frequently misclassified by the single lists.
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